Voltammetric Determination of Flunixin on Molecularly Imprinted Polypyrrole Modified Glassy Carbon Electrode.

Abd-Elgawad Radi,Tarek Wahdan,Nadia Abd El-Ghany

doi:10.1155/2016/5296582

Abd-Elgawad Radi, Tarek Wahdan + Show 1 more

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https://doi.org/10.1155/2016/5296582

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Abstract

A novel electrochemical sensing approach, based on electropolymerization of a molecularly imprinted polypyrrole (MIPpy) film onto a glassy carbon electrode (GCE) surface, was developed for the detection of flunixin (FXN). The sensing conditions and the performance of the constructed sensor were assessed by cyclic, differential pulse and (DPV) square wave voltammetry (SWV). The sensor exhibited high sensitivity, with linear responses in the range of 5.0 to 50.0 µM with detection limits of 1.5 and 1.0 µM for DPV and SWV, respectively. In addition, the sensor showed high selectivity towards FXN in comparison to other interferents. The sensor was successfully utilized for the direct determination of FXN in buffalo raw milk samples.

Highlights

Flunixin (2-[[2-methyl-3-(trifluoromethyl)phenyl]amino]pyridine-3-carboxylic acid) (FXN) (Scheme 1) is a nonsteroidal anti-inflammatory drug (NSAID)
FXN has been shown to improve the clinical effects seen after experimentally induced coliform mastitis in the goat [9] and has a beneficial effect on the course of experimentally induced bovine pneumonic pasteurellosis (Pasteurella haemolytica, biotype AI) [5]
Cyclic voltammetry revealed the absence of reverse wave/peak at any i i

Summary

Introduction

Flunixin (2-[[2-methyl-3-(trifluoromethyl)phenyl]amino]pyridine-3-carboxylic acid) (FXN) (Scheme 1) is a nonsteroidal anti-inflammatory drug (NSAID). The major effect of FXN, as all the NSAIDs, is the nonselective reversible inhibition of both isoforms of cyclooxygenase, COX, an enzyme responsible for inflammation and pain via catalysis the formation of prostaglandins through the arachidonic acid cascade [1]. It is widely used in veterinary medicine for the treatment of various diseases in several animal species. FXN has been shown to improve the clinical effects seen after experimentally induced coliform mastitis in the goat [9] and has a beneficial effect on the course of experimentally induced bovine pneumonic pasteurellosis (Pasteurella haemolytica, biotype AI) [5]. FXN, in conjunction with standard antimicrobial therapy, may be of potential value in the treatment of the infection with P. haemolytica, common infection in sheep flocks [10]

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