Voltammetric Determination of Carcinogenic Derivatives of Pyrene Using a Boron-Doped Diamond Film Electrode

Abstract

Based on the study of voltammetric behavior of carcinogenic 1-nitropyrene (1-NP), 1-aminopyrene (1-AP), and 1-hydroxypyrene (1-HP), optimum conditions have been found for the determination of these analytes by differential pulse voltammetry (DPV) at a boron-doped diamond film electrode. The optimum medium was methanol-Britton–Robinson buffer (BR buffer) pH 3.0 (70:30) for 1-NP and 1-AP, and methanol-BR buffer pH 5.0 (70:30) for 1-HP. Concentration dependences of the DPV response were measured in the range 1 · 10−6–1 · 10−4 mol dm−3 (R = −0.9998) with the limit of detection (LOD) 3 · 10−7 mol dm−3 for 1-NP, 1 · 10−7–1 · 10−5 mol dm−3 (R = 0.9971) with LOD 6 · 10−8 mol dm−3 for 1-AP, and 1 · 10−7–1 · 10−5 mol dm−3 (R = 0.9934) with LOD 1 · 10−7 mol dm−3 for 1-HP. Simultaneous determination of 1-NP and 1-AP in a mixture was tested in the methanol-BR buffer pH 3.0 (70:30) medium as well. The content of 1-AP in the concentration range from 1 · 10−6 to 1 · 10−4 mol dm−3 had no effect on the sensitivity of the determination of 1-NP, and vice versa. Due to the close peak potentials of 1-AP and 1-HP, the direct determination of their mixture using voltammetric methods is impossible.