Abstract
Poly (xylenol orange) film-coated graphite pencil electrode was fabricated for the detection of dopamine in the presence of ascorbic acid and uric acid in phosphate buffer solution of pH 7. The redox peaks obtained at modified electrode shows a good enhancement. The scan rate effect was found to be a diffusion-controlled electrode process. The electrochemical oxidation of dopamine was depended on pH, and the limit of detection was found to be 9.1×10−8 M. The simultaneous study gave and excellent result with great potential difference between dopamine and other bioactive organic molecules by using both cyclic voltammetric and differential pulse voltammetric techniques. The present modified graphite electrode was applied to the detection of dopamine in the injection samples, and the recovery obtained was satisfactory.
Highlights
The graphite pencil electrode (GPE) has been successfully acting as a biosensor in modern electroanalytical field
The aim of our work was to fabricate stable electrode by electropolymerising xylenol orange (The structure of xylenol orange was shown in Scheme 1) on the surface of graphite pencil electrode to achieve the challenge of simultaneous determination of DA in the presence of ascorbic acid (AA), and uric acid (UA) in physiological pH
The pencil-lead rods were (HB 0.5 mm in diameter and 6 cm length) purchased from local bookstore. 25 × 10−3 M xylenol orange stock solution was prepared in double-distilled water. 10 × 10−5 M DA stock solution was prepared in 0.1 M perchloric acid solution. 10 × 10−3 M UA stock solution was prepared in 0.1 M NaOH and 10 × 10−3 M AA was prepared in double-distilled water
Summary
The graphite pencil electrode (GPE) has been successfully acting as a biosensor in modern electroanalytical field. The aim of our work was to fabricate stable electrode by electropolymerising xylenol orange (The structure of xylenol orange was shown in Scheme 1) on the surface of graphite pencil electrode to achieve the challenge of simultaneous determination of DA in the presence of AA, and UA in physiological pH. In this investigation electrochemical method was chosen for the detection of DA because, the electrochemical methods have proved to be selective, sensitive, reliable, and cost effective [25]. This work discussed about sensitivity, selectivity and reproducibility of neurotransmitter at poly(XO)/GPE at physiological pH
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