Voltage-gated channels involved in taste responses and characterizing taste bud cells in mouse soft palates

Abstract

Taste bud cells (TBCs) on soft palates differ from those on tongues in innervation and chemosensitivity. We investigated voltage-gated channels involved in the taste responses of TBCs on mouse soft palates under in-situ tight-seal voltage/current-clamp conditions. Under the cell-attached mode, TBCs spontaneously fired action currents, which were blocked by application of 1 μM TTX to TBC basolateral membranes. Firing frequencies increased in response to taste substances applied to TBC receptor membranes. Under the whole-cell clamp mode, as expected, TBCs produced various voltage-gated currents such as TTX-sensitive Na + currents ( I Na), outward currents ( I out) including TEA-sensitive and insensitive currents, inward rectifier K + currents ( I ir), and Ca 2+ currents including T-type, P/Q-type, and L-type Ca 2+ currents. We classified TBCs into three types based on the magnitude of their voltage-gated Na + currents and membrane capacitance. HEX type (60% of TBCs examined) was significantly larger in Na + current magnitude and smaller in membrane capacitance than LEX type (23%). NEX type (17%) had no Na + currents. HEX type was equally distributed within single taste buds, while LEX type was centrally distributed, and NEX type was peripherally distributed. There were correlations between these electrophysiological cell types and morphological cell types determined by three-dimensional reconstruction. The present results show that soft palate taste buds contain TBCs with different electrophysiological properties, and suggest that their co-operation is required in taste transduction.