Abstract
Nanopore-based biosensing has attracted more and more interests in the past years, which is also regarded as an emerging field with major impact on bio-analysis and fundamental understanding of nanoscale interactions down to single-molecule level. In this work, the voltage-driven translocation properties of goat antibody to human immunoglobulin G (IgG) are investigated using nanopore arrays in polycarbonate membranes. Obviously, the background ionic currents are modulated by IgG molecules for their physical place-holding effect. However, the detected ionic currents do ‘not’ continuously decrease as conceived; the currents first decrease, then increase, and finally stabilize with increasing IgG concentration. To understand this phenomenon, a simplified model is suggested, and the calculated results contribute to the understanding of the abnormal phenomenon in the actual ionic current changing tendency.
Highlights
In recent years, the new generation of analytical technology based on nanopores or nanochannels provides possibilities for low-cost and rapid biosensing and DNA sequencing
When a certain voltage is applied to the two liquid cells through Pt electrodes, K+ and Cl− are driven to pass through nanopores, which result in certain background ionic currents
It is obvious that bigger voltage corresponds to bigger electrostatic force, which will accelerate the movements of K+ and Cl− and will lead to rather bigger ionic currents
Summary
The new generation of analytical technology based on nanopores or nanochannels provides possibilities for low-cost and rapid biosensing and DNA sequencing. It is regarded as an emerging field which is expected to have major impact on bio-analysis and fundamental understanding of nanoscale interactions down to single-molecule level. More and more theoretical and experimental work aiming to understand and design nanopore-based devices have been done, which is at the forefront of life science, chemistry, material science, and (bio) physics. The scheme of nanofluidic analytical device (Figure 1) based on nanopores for biomolecular sensing can be depicted as following: two separated liquid cells with certain electrolyte are linked by nanopores; along the length direction of nanopore, certain voltage is applied, which results in background ionic current. Resistive-pulse sensing can be used for the detection and characterization of ions and biopolymers [19-23]
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