Abstract
The voltage-dependent anion channel (VDAC) is the major transport protein in the outer membrane of mitochondria and plays crucial roles in energy metabolism, apoptosis, and metabolites transport. In plants, the expression of VDACs can be affected by different stresses, including drought, salinity and pathogen defense. In this study, we investigated the expression pattern of AtVDAC2 in A. thaliana and found ABA suppressed the accumulation of AtVDAC2 transcripts. Further, phenotype analysis of this VDAC deregulated-expression transgenic Arabidopsis plants indicated that AtVDAC2 anti-sense line showed an ABA-insensitivity phenotype during the early seedling development under ABA treatment. The results suggested that AtVDAC2 might be involved in ABA signaling in A. thaliana.
Highlights
The voltage-dependent anion channel (VDAC) is the major channel on the outer membrane of mitochondria
Further phenotype analysis of the stable AtVDAC2 transgenic plants confirmed that AtVDAC2 involved in Abscisic acid (ABA) signaling
The relative AtVDAC2 abundance was detected by semi-quantitative RT-polymerase chain reaction (PCR)
Summary
The voltage-dependent anion channel (VDAC) is the major channel on the outer membrane of mitochondria. Earlier studies on VDAC mainly focused on its isoforms in animals and yeasts [4,5]. Studies using Saccharomyces cerevisiae mutants depleted of either isoform of VDAC, showed that both the cytosol and mitochondria redox states depend on the presence of VDAC [7]. The researches on plants mainly focus on the identification and the expression pattern analysis of the VDAC isoforms. The expression pattern analysis revealed that VDAC affected plant response to different stresses, including drought, heat shock, salinity [11,14], as well as defense against pathogen [12]. Using the yeast two-hybrid system, our earlier studies have revealed that one isoform of AtVDACs, AtVDAC2 (At5g67500), is a potential protein interaction partner of one ABA signal component, which is an interaction partner of ABI1 and ABI2. Further phenotype analysis of the stable AtVDAC2 transgenic plants confirmed that AtVDAC2 involved in ABA signaling
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