Volatiles from Marina neglecta: Biocide effect on insect vectors of tropical diseases in Southern Mexico

Abstract

• Essential oils of M. neglecta contained β-pinene, β-caryophyllene and germacrene D. • Essential oils and main volatiles produced a biocide effect in four insect vectors. • β-Pinene produced significant and fast mortality in life stages of triatomines. • Germacrene D produced significant and fast mortality in life stages of mosquitoes. Preventive measures based in the control of insect vectors are considered as the best choice to decrease the incidence of insect-borne diseases. Herein we report on the volatile content of the leaf essential oils from Marina neglecta , a medicinal plant distributed in the tropical regions of southern Mexico. In order to investigate the chemical variation of the essential oils, a volatile screening was performed during the four seasons of the years 2016–2019. Simultaneously, their biological activity was tested on distinct life stages of Meccus pallidipennis , M. bassolsae , Aedes aegypti and A. albopictus . Essential oils were mainly constituted of β-pinene (>30%) β-caryophyllene (>25%) and germacrene D (>13%). Dorsal-abdomen application of essential oils on triatomines, revealed an efficient LC 50 for nymphs of the stages I to III (4 µg/insect), nymphs of the stages IV to V (5–6 µg/insect), and adults (7–8 µg/insect). The LT 50 for the stages I to III was between 6 and 8 h, whereas that for the stages IV to V and adults oscillated between 12 and 16 h and 22 to 26 h, respectively. Fumigation experiments performed on nymph V, demonstrated that 300 µg L −1 air produced 100% mortality after 72 h post-treatment. Among tested volatiles, β-pinene and β-caryophyllene produced a comparable mortality rate ( p < 0.01) than that of essential oils in the stages assayed. Essential oils showed strong larvicidal (LC 50 , 24–36 µg mL −1 ) and adulticidal (35–48 µg mL −1 ) activities in mosquito species with an LT 50 of 4.5 h and 25–35 min, respectively. The evaluation of β-pinene produced a significant mortality rate ( p < 0.01) in larvae whereas germacrene D was the most effective volatile ( p < 0.01) against adults of both mosquito species. According to our results, β-pinene was the most effective volatile against the four insect species evaluated and its effect was comparable to that of the essential oil.