Abstract
In the present study, three strains each of Bacillus subtilis and Trichoderma harzianum were compared for their antagonism against Fusarium oxysporum f. sp. lycopersici (FOL), responsible for causing wilt disease in tomato plant. In vitro antifungal volatile assay revealed that B. subtilis 2274 caused significantly (p 75%) were evaluated by profiling of volatile organic compounds (VOC). The emitted volatiles were extracted by non-invasive solid phase micro extraction (SPME) paired with gas chromatography-mass spectrometry (GC–MS). In total, 48 VOC were identified from B. subtilis 2274, and 20 from T. harzianum 3928. This study documented the VOC profile of efficient bacterial and fungal biocontrol strains mentioned above through a robust analytical method. It could successfully correlate the higher potential of B. subtilis as an antagonist as compared to T. harzianum with production of additional compounds of antifungal categories including derivatives of pyran, naphthalene and morpholine.
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