Abstract
The tuberose, Polianthes tuberosa L., is in the family Agavaceae. Its scent is very sweet and is used in perfumery as a source of essential oil and aroma compound. Therefore, the objective of this study was to investigate the emission profile of volatile compounds in different floral developing stages and diurnal times of the day to find the best condition for volatile releasing patterns in tuberose flowers. The flowers were divided into three stages, bud, half-full bloom and full bloom, and incubated in a small vial (25 ml) for 30 min at 25°C. The chemical composition of the tuberose was analyzed by gas chromatography-mass spectrometry (GC-MS) every 6 h (started at 9 am, 3 pm, 9 pm and 3 am) for one day using SPME fiber 65 µm, PDMS-DVB coating. The results showed that the main chemical components detected in fresh flowers were methyl benzoate, eucalyptol, methyl salicylate, and methyl antharilate in all stages of flowers. Isopropanoid and phenylpropanoid pathways were the main volatile biosynthesis in the flowers. The more mature the flower, the higher concentration of the volatile compounds. Ester compounds produced highly in full bloom flowers and during night time. The quality and quantity of volatile compounds at 9 pm and 3 am were higher while the lowest amount of volatiles was found at 3 pm. The proper condition for volatile release in tuberose flowers was in full bloom flowers at 3 am.
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