Abstract
Cold storage is important for extending the shelf life of Lentinula edodes; however, the mechanism underlying aroma changes during cold storage is not understood. In this study, we analyzed the quality, volatile organic compounds, volatile precursors, and related enzyme activities of Lentinula edodes during cold and room temperature storage. The transcriptome was analyzed to determine the expression of aroma-related genes in Lentinula edodes. The results showed that cold storage maintained a better appearance and aroma profile than room-temperature storage. Fifteen volatile organic compounds were identified as potential biomarkers for monitoring the aroma changes in Lentinula edodes. Transcriptomic results revealed that lipoxygenase-related genes were important for regulating 1-octen-3-ol biosynthesis, whereas the three upregulated genes were related to terpenoid degradation. This study provides new insights into regulating aroma metabolism in Lentinula edodes during cold storage.
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