Abstract

Myrtaceae family includes many species with taxonomic challenges, making it one of the most complex families to identify. This study used DNA barcoding to find molecular markers for species authentication based on the Myrtaceae family's chemical composition and genetic diversity. Essential oils and genetic material were extracted from the leaves of six different species:Eugenia uniflora,E. patrisii,Myrcia splendens,Psidium guajava,P. guineense, andPsidiumsp. The samples were analyzed based on compound classes and grouped into two categories. Group I included samples with high amounts of oxygenated sesquiterpenes (3.69-76.05%) and fatty acid derivatives (0.04-43.59%), such asE. uniflora,Myrcia splendens, andE. patrisii. Group II included samplesP. guajava,P. guineense, andPsidiumsp., which had a significant content of monoterpene hydrocarbons (0.69-72.35%), oxygenated sesquiterpenes (8.06-68.1%), phenylpropanoids (0.45-22.59%), and sesquiterpene hydrocarbons (0.27-21.84%). ThePsbA-trnHgene sequences had a high genetic variability, allowing the species to be distinguished. A phylogenetic analysis showed two main clusters with high Bootstrap values corresponding to the subtribes Eugeniineae, Myrciinae, and Pimentinae. The results suggest a weak correlation between genetic and chemical data in these Myrtaceae species.

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