Volatile organic compounds of Hanseniaspora uvarum increase strawberry fruit flavor and defense during cold storage.

Luyao Wang,Guoxia Dou,Hongna Guo,Qiuqing Zhang,Hongmei Xiao,Wei Yu,Xiaojie Qin,Chunhao Jiang

doi:10.1002/fsn3.1116

Luyao Wang, Guoxia Dou + Show 6 more

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https://doi.org/10.1002/fsn3.1116

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Abstract

Volatile organic compounds (VOCs) of antagonistic yeasts are considered as environmental safe fumigants to promote the resistance and quality of strawberry (Fragaria ananassa). By GC‐MS assays, VOCs of Hanseniaspora uvarum (H. uvarum) fumigated strawberry fruit showed increased contents of methyl caproate (5.8%), methyl octanoate (5.1%), and methyl caprylate (10.9%) in postharvest cold storage. Possible mechanisms of H. uvarum VOCs involved in regulations of the defense‐related enzymes and substances in strawberry were investigated during postharvest storage in low temperature and high humidity (2 ± 1°C, RH 90%–95%). Defense‐related enzymes assays indicated H. uvarum VOCs stimulated the accumulation of CAT, SOD, POD, APX, PPO, and PAL and inhibited biosynthesis of MDA in strawberry fruit under storage condition. Moreover, the expression levels of related key enzyme genes, such as CAT, SOD, APX42, PPO, and PAL6, were consistently increased in strawberry fruit after H. uvarum VOCs fumigation.

Highlights

Fungal infections bring serious loss in strawberry production, especially during field and postharvest phases (Paulus, 1990)
These results obviously showed that the H. uvarum volatile organic compounds (VOCs) treatment can maintain the quality of strawberry during cold storage period
Our results proved that VOCs produced by H. uvarum could significantly induce the accumulation of defense‐related enzymes, such as superoxide dismutase (SOD), POD, CAT, APX, phenylalanine ammonialyase (PAL), and polyphenol oxidase (PPO)

Summary

Introduction

Fungal infections bring serious loss in strawberry production, especially during field and postharvest phases (Paulus, 1990). There is much concern about fungicide residue in agricultural product, and biocontrol has been considered as a more acceptable method for controlling postharvest diseases (Karunaratne, 2011; Kilani‐Feki et al, 2016; Mohamed & Saad, 2009; Pretorius, Van, & Clarke, 2015). Multiple biocontrol agents (BCAs) have been isolated from environment, and they were shown to facilitate the control of postharvest decay of strawberry fruit (El Ghaouth & Wilson, 2002; Menel, Faten, & Moktar, 2012; Wei, Mao, & Tu, 2014; Zhang et al, 2007), suggesting possible application of BCAs in strawberry postharvest storage. It was reported that VOCs treatment may control postharvest fungal disease and increase the storage period of strawberry fruit (Archbold, Hamiltonkemp, Barth, & Langlois, 1997)

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