Volatile constituents of Origanum vulgare L., ‘thymol’ chemotype: variability in North India during plant ontogeny

Abstract

Essential oils derived from six different phenophases, namely early vegetative stage, late vegetative stage, early flowering stage, full flowering stage (FFS), late flowering stage and seed shattering stage of Origanum vulgare L. grown in Kumaon region of Uttarakhand, India were investigated by GC and GC-MS. A total of 38 constituents, representing 97.4–99.7% of the total oil composition, were identified. Major components of oils were thymol (40.9–63.4%), p-cymene, (5.1–25.9%), γ-terpinene (1.4–20.1%), bicyclogermacrene (0.2–6.1%), terpinen-4-ol (3.5–5.9%), α-pinene (1.6–3.1%), 1-octen-3-ol (1.4–2.7%), α-terpinene (1.0–2.2%), carvacrol (<0.1–2.1%), β-caryophyllene (0.5–2.0%) and β-myrcene (1.2–1.9%). Thymol, terpinen-4-ol, 3-octanol, α-pinene, β-pinene, 1,8-cineole, α-cubebene and (E)-β-ocimene were observed to be higher during FFS. The study showed that plant stage had a significant effect on the essential oil content and composition of O. vulgare grown in the hilly tracks of Northern India.