Abstract
Moroccan honey with Bupleurum spinosum (Apiaceae/Umbelliferae) as the main pollen source is locally known as Zantaz honey. In the present work, the volatiles from 18 honeys of this type, were isolated by hydrodistillation and analysed by Gas Chromatography (GC) and Gas Chromatography-Mass Spectrometry (GC-MS), for volatiles quantification and identification. The percentage composition of the volatiles and pollen profiling was used to determine the relationship between the different samples by cluster analysis. Two poorly correlated clusters were defined in volatiles analysis. Cluster A, with 17 out of the 18 samples, included four subgroups dominated by straight-chain hydrocarbons and fatty acids in variable proportions. Cluster B included just one sample, with 1-phenyldodec-1-en-3-one as the main component. Mono- and sesquiterpene hydrocarbons, as well as oxygen-containing mono- and sesquiterpenes occurred always <2%. Two moderately correlated clusters were defined after pollen profiling cluster analysis. Pollen Cluster A included 17 samples having in common the presence of B. spinosum and Populus sp. pollen. The one sample from Cluster B was moderately correlated with Cluster A and showed the lowest percentage of B. spinosum pollen. Pollen volatiles analysis would be relevant in assessing the presence of putative pollen volatile marker compounds and the importance of pollen profile on honey volatiles.
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