Abstract
CRYOPRESERVATION by vitrification is a new ultralow-temperature-storage technique developed in the 1980s. Operationally, procedures for vitrification of biological specimens consist of the following basic steps: treatment of the samples in a highly concentrated vitrification solution and then plunging the specimens into liquid nitrogen. The word vitrification is sometimes used to describe the high moisture of the in vitro cultures or regenerants. However, in this note, this word means a biophysical process in which the cells and the solution are transformed into a homogeneous and amorphous state. The water in cells does not change into ice, but in a...
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