Abstract
ABSTRACTThe aim of our study was to test four vitrification methods for preservation of mice embryos, in different developmental stages, using 6 mol/l glycerol and 20% sucrose as vitrification media. Morula stage embryos were vitrified with OPS straws or classic straws using two equilibration steps, with a hatching rate of 50% and 47.62% respectively. Blastocyst stage embryos survived better in 0.25 ml straws with three equilibration steps, 36.84% hatching rate. Two-cell embryos are sensitive to freezing, but satisfactory results could be obtained with OPS vitrification, hatching rates of 22.73%. The success of the vitrification technique depends on the developmental stage of the embryo and the method used.
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