Abstract
Isolated blastomeres obtained by embryo biopsy serve mainly for preimplantation genetic screening. Blastomeres are undifferentiated embryonic cells that include all the embryo genetic information. A lot of developing technologies may benefit by the efficient cryopreservation of blastomeres for future potential use, especially for stem cell culture and differentiation control. We are hereby reporting for the first time the feasibility of preserving individual isolated blastomeres in microvolumes in a closed vitrification system. Using a cryotip and propagation in microvolumes, isolated mice blastomeres were vitrified and warmed with 100% post-warming survival.
Highlights
Blastomeres can be isolated from cleavage stage embryos by embryo biopsying
This technique is used for preimplantation genetic diagnosis (PGD) of inherited diseases, or pre-implantation genetic screening (PGS) for aneuploidy
Despite the increasing applications of closed vitrification devices, there has been no description in the literature as of on the use of a closed vitrification system to vitrify isolated blastomeres
Summary
Published: 19 February 2009 Reproductive Biology and Endocrinology 2009, 7:17 doi:10.1186/1477-7827-7-17
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