Abstract
To study the impact of vitrification of in vitro matured caprine oocytes on in vitro fertilization (IVF), ovaries were collected from a local abattoir and goat oocytes aspirated. The oocytes were subjected to in vitro maturation (IVM) for 27 h. Twenty-seven hours post IVM oocytes were cryopreserved in a vitrification solution (VS) consisting of propylene glycol (40%, w/v) and trehalose (0.25 mol l −1) in PBS with 4% (w/v) BSA. Some oocytes were exposed to propylene glycol without cryopreservation to check the degree of damage induced by propylene glycol. In the vitrified group, 94.3% of the oocytes were viable for IVF. 16.1% of the oocytes were damaged after thawing. From the vitrified group, 75.8% of the oocytes were subjected to IVF, while for 18 h. Cytological studies following fixing of the IVF oocytes revealed 17.37% of the oocytes showing a pronucleus in the control group, compared to 8.08 and 7.86% in the vitrified and cryoprotectants exposed groups, respectively. This study revealed that goat oocytes can be successfully cryopreserved using a simple vitrification method. However, the fertilizing ability of oocytes was severely affected by both vitrification and exposure to cryoprotectants.
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