Abstract
Clones of vitrified Lemna minor plants were recovered following heat and osmotic shock. Their growth in vivo was about two-thirds as rapid as glaucous plants, and they contained one-third as much chlorophyll. Reproduction of the vitrified state of plants in vivo was nearly complete over three fronddoublings in the presence of 0.5 mg · L -1 isopentenyladenine (2iP), and vitrification was lost immediately in the absence of 2iP. The correlation between in vivo maintenance of the vitrified plant state and cytokinin concentration was 0.989.
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