Abstract
BackgroundThis study aims to carry out the vitrification freezing of a large ovarian tissue in the human body, and evaluate its feasibility.ResultsA total of 18 ovarian tissues in the human body were selected, and each tissue was cut into three large ovarian cortex slices. These tissues were randomly divided into three groups: vitrification freezing group (group A), programmed freezing group (group B), and fresh control group (group C). Then, the morphological analysis and apoptosis detection of each ovarian tissue was carried out, followed by the recycling of ovarian tissues at three weeks after the heterotransplantation of nude mice, in order to detect the follicle preservation conditions. The immunohistochemistory method was applied to detect the follicle activity. In comparing the proportion of primordial follicle with normal morphology after unfreezing between group A and group B, the difference was not statistically significant (P > 0.05). Furthermore, the incidence of follicle apoptosis in group A and group B was higher than that in the group C (P < 0.05). However, when comparing between group A and group B, the difference was not statistically significant (P > 0.05). The interstitial cell apoptosis rate in group A was lower than that of the group B, showing that the difference was statistically significant (P < 0.05).ConclusionsCompared with programmed freezing, the vitrification freezing of large ovarian tissues in the human body was feasible to a certain extent. This can be used as an alternative scheme to realize the freeze preservation of ovarian tissues in the human body.
Highlights
This study aims to carry out the vitrification freezing of a large ovarian tissue in the human body, and evaluate its feasibility
There were some problems after tumor radiotherapy and chemotherapy, such as ovarian dysfunction and dysgenesis, which has become a top concern for them [1]
The average age of these patients ranged within 37.9 ± 6.4 years old, and these patients had no endocrine disease and hormone drug administration history, or chemotherapy and radiotherapy history
Summary
This study aims to carry out the vitrification freezing of a large ovarian tissue in the human body, and evaluate its feasibility. The progress of tumor treatment technology has greatly improved the long-term prognosis of malignant tumor patients. More and more females at childbearing age and juvenile female patients have survived from this. The vitrification cryopreservation of ovarian tissues is a kind of new technology secondary to embryo freezing and oocyte cryopreservation, with the potential for preserving female reproductive function. Through the quick cooling rate, it can be used to induce internal and external cells to the vitrification state, avoiding the formation of ice crystals.
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