Vitreoscilla Hemoglobin Geni (vgb) Taşıyan İki Farklı Plazmidin Citrobacter freundii’nin ATP Üretimi ve Oksijen Alınımına Etkileri

Abstract

In this study, the use of recombinant oxygen uptake system on production of ATP production, oxygen uptake and cell mass was investigated. Citrobacter freundii and their vgb recombinants were used. Citrobacter freundii, a broad host range plasmids Cf[pMK 79] and Cf[pUC 8:15] was used. Best ATP production by Cf[pMK 79] (0.17 mg/ g). C f[pMK 7 9] s train t he l evel o f o xygene u ptake w as h igher t han C f[pUC 8 :15]. Whereas Cf[pMK 79] the OD600 values have been falling at the end of 48 hours.