台灣細本葡萄(Vitis thunbergii Sieb. & Zucc.)收集系有效成分變異性之研究

Abstract

Resveratrol, piceid, kaempferol, and four flavonoid glycosides are active compositions present in Vitis thunbergii Sieb. & Zucc. In this study, 53 collections collected from Taiwan were used as materials to investigate the variations of active compositions among collections, seasons, and plant parts, the correlation among active compositions of various plant parts were analyzed. The results are summarized as follows： During the cultivation period of V. thunbergii, resveratrol, kaempferol, and four flavonoid glycosides accumulate at the highest levels from December to February during the growth period. Significant differences are found in resveratrol, piceid, kaempferol, and flavonoid glycosides contents of harvest months, collections, plant parts, and the interactions among them by combined ANOVA analysis. Means of the active compositions contents in all plant parts were higher in March than in August. Resveratrol and piceid contents show the highest levels in root and stem. Kaempferol and four flavonoid glycosides are found to be the highest levels in leaf following with young stem. Few flavonoids can be detected in root and stem. Significant differences are found in plant ages, plant parts, and the interaction of plant ages and plant parts of V. thunbergii for resveratrol, piceid, kaempferol, and flavonoid glycosides contents. The 3-year-old V. thunbergii has the highest levels of resveratrol and piceid and the 1-year-old plants show the highest levels of kaempferol and flavonoid glycosides contents, respectively. Significant positive correlations of resveratrol among the root, stem, and young stem are found by correlation analyses. Piceid, kaempferol, and four flavonoid glycosides show significant correlation between leaf and young stem. Resveratrol content of root, stem, and leaf are positively correlated with piceid. Hyperin content of stem shows positive correlation with isoquercitrin and quercitrin, and hyperin content of leaf also shows positive correlation with quercitrin. In leaf, isoquercitrin is positive correlated with rutin but negative correlated with quercitrin. According to this study, high contents of resveratrol in stem and root, and flavonoids in leaf and young stem can be improved by selection.