評估VITEK 2 Compact及BD Phoenix自動化微生物分析儀使用於臨床微生物實驗室的可行性

Abstract

A comparative study was conducted to evaluate the performance of identification (ID) and antimicrobial susceptibility testing (AST) between the VITEK 2 Compact (V2C) and BD Phoenix automated microbiology systems. A total of 124 isolates that were originated from external proficiency testing strains were used to test for ID accuracy. Another 61 reference strains with known antimicrobial susceptibility were kindly provided by the BD company and were used to test for AST accuracy. The correctness of AST results were determined according to the BD database. The ID accuracy at the genus level was the same for both instruments (98.4%), with only two isolates incorrectly identified. However, at the species lever, the ID accuracy was 96.8% and 93.6% for V2C and Phoenix, respectively. As to the AST results, the overall essential agreement/categorical agreement for all Gram-negative bacilli tested were 98.3%/97.7% and 99.4%/98.7% for V2C and Phoenix, respectively. No significant difference was found in the rates of major error and very major error between the two systems. For staphylococci and enterococci, the essential agreement and categorical agreement were 96.0% and 94.6% for V2C, and both 98.9% for Phoenix. The rates of major error and minor error were 3.4% and 2.5% for V2C, while no such errors were found for Phoenix. The rates of very major error were both 3.5% for the two systems. Through the analysis, both systems demonstrated adequate performance to be used for the ID and AST in a clinical microbiology laboratory.