Abstract
This review describes the application of thin-layer chromatography (TLC) and high-performance thin-layer chromatography (HPTLC) in the separation and identification of vitamins from their mixtures using different kinds of mobile phases such as isocratic and mixed solvents as well as mobile phases containing various modifiers. TLC has long been used for the separation and semiquantitative determination of biomolecules including vitamins. HPTLC is the modified version of TLC, and it offers the advantages of better resolution, smaller sample size, and lesser volumes of mobile phases. In recent years, the emergence of advanced and fully automated chromatographic techniques such as high-performance liquid chromatography (HPLC) and gas chromatography (GC) has reduced the application of TLC/HPTLC for vitamins analysis. However, due to the inherent simplicity and cost-effectiveness, both TLC and HPTLC continue to be the methods of choice in small laboratories of developing countries. In this short review, the literature survey on TLC/HPTLC of vitamins covering the period 2011‒2019 has been encapsulated in a tabular form.
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