Vitamins B therapy in Regeneration of Peripheral Neuropathy Associated with Lipid Profile

Abstract

Objective: To investigate the role of serum lipid profile during the regeneration of experimental crush peripheral nerve injury to rats. Besides, to investigate which type of vitamin B therapy is better to accelerate the regeneration processes.Methods: Seventy-five male albino rats male, aged between 10 to 12 weeks were equally divided into 5 treated groups: B1, B6, B12, Tri-B complex and normal saline. Rats of therapeutic groups were administered according to the grouping and lasted to 45 consecutive days, and every 5 rats were sacrificed by euthanizing at 15th, 30th and 45th days. At dissection, sciatic nerve samples are taken from the site of crushed lesion and immersed in fixed formaldehyde 10%. 05 to 7 µm thick paraffin sections were stained by H&E and blood sample for lipid profile analysis.Results: The result showed no significant differences in serum lipid profile between therapeutic groups and control was found. Histological changes were shown on 15th days as, diffuse degeneration of the myelinated fibers with a marked vacuolization of some nerve fiber, numerous atrophic disoriented fibers with a large number of mononuclear phagocytes infiltration and degenerative debris. On 45th days there is a clear normal orientation of collagen fiber with very little debris and no vacuolization of the nerve. Morphometric measurements showed the myelin sheath thickness was increased significantly on the 30th and 45th days as compared with the control group. The result showed vitamin B12 is better in acceleration to nerve regeneration B12 groups showed better regenerative rate among other groups.Conclusion: no changes in serum lipid profile on experimental animals during regeneration processes. Vitamin B12 is better for enhancing the regenerative process.