Vitamin E-added Highly Cross-Linked Polyethylene Decreases the Risk of Osteolysis in an In Vivo Arthroplasty Model.

Abstract

Introduction Aseptic loosening is one of the most important complications of arthroplasty surgery. It is known that immune response against particles plays role in the pathogenesis of aseptic loosening. Polyethylene (PE) has an important place in these particles. There are limited in vivo studies examining aseptic loosening caused by PE residues. Objective The aim of the present study is to evaluate the aseptic loosening created by highly cross-linked PE (HXLPE) and vitamin E-added PE particlesin an in vivo knee prosthesis model. Materials and methods Thirty-nine male Sprague-Dawley rats, which were randomized into three groups, were included in the study. After surgical exposure of knee joints of rats, femoral intramedullary canals were drilled and instilled with isolated saline solution and saline solution that contained standard PE or vitamin E-added PE particles according to their groups. Afterwards, a titanium implant was placed on the femoral articular surface of each animal. Rats received intraarticular injections weekly of the same solution, which was initially instilled into their femoral canal. The rats were sacrificed at the end of the third week and then underwentradiological and histopathological evaluations. Result In histopathological evaluation, periprosthetic membrane formation, inflammatory cell change, and cellular damage of cartilage and bone tissue around the implant were assessed. There was a statistically lesser amount of cellular damage and periprosthetic membrane formation in the vitamin-E/HXLPE group compared to the HXLPE group (p=0.04, p=0.001). No significant difference was found between the PE groups with respect to inflammatory cells (p=0.715). Conclusions HXLPE caused more significant osteolysis compared to VE-HXLPE. Antioxidants in PE could provide a reduction in osteolysis and aseptic loosening.