Vitamin d-binding protein levels in plasma and gingival crevicular fluid of patients with generalized aggressive periodontitis.

Xin Zhang,Zhibin Chen,Li Xu,Huanxin Meng,Li Zhang,Ruifang Lu,Dong Shi,Xianghui Feng

doi:10.1155/2014/783575

Abstract

Vitamin D-binding protein (DBP) is the main transport protein of vitamin D and plays an important role in the immune system and host defenses. The purpose of this study was to measure DBP levels in plasma and gingival crevicular fluid (GCF) of patients with generalized aggressive periodontitis (GAgP), in comparison to healthy controls, with the goal of elucidating the relationship between DBP and GAgP. Fifty-nine GAgP patients and 58 healthy controls were recruited for the study; clinical parameters of probing depths (PD), bleeding index, and attachment loss (AL) were recorded. DBP levels were measured by enzyme-linked immunosorbent assay. From the results, GAgP patients had higher plasma DBP concentrations (P < 0.001) but lower GCF DBP concentrations (P < 0.001) than healthy controls. In GAgP group, after controlling the potential confounders of age, gender, smoking status, and BMI index, GCF DBP concentrations correlated negatively with PD (P < 0.001) and AL (P = 0.009). Within the limits of the study, we concluded that decreased GCF DBP level and increased plasma DBP level are associated with periodontitis.

Highlights

Periodontitis is primarily a bacterial infection caused by a diverse group of microorganisms [1]
Fifty-eight periodontal healthy controls were selected from staff and students at the School of Stomatology; none of them had any clinical evidence of periodontitis (PD ≤ 3 mm; the percentage of sites with bleeding on probing (BOP) was
The mean probing depths (PD) of the 44 periodontitis sites selected for gingival crevicular fluid (GCF) sampling was 6.70 ± 1.89 mm, with a range from 4 mm to 10 mm

Summary

Introduction

Periodontitis is primarily a bacterial infection caused by a diverse group of microorganisms [1]. Generalized aggressive periodontitis (GAgP) is a subtype of periodontitis that mainly affects younger patients and is characterized by episodic and rapid loss of periodontal supporting tissues [2]. Though microorganisms are considered to be the etiologic agent that causes this inflammatory lesion, it is the chemical mediators of inflammation that play a pivotal role in the loss of periodontal connective tissue, as well as supporting alveolar bone [3]. Vitamin D has been shown to have immunomodulatory effects in vitro and in animal studies. Previous research from our group revealed a relationship between 25-hydroxyvitamin D (25[OH]D) and GAgP. We reported that plasma 25(OH)D levels were higher in patients with

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