Vitamin D Supplementation Combined With Aerobic Training Increases the Gene Expression of Antioxidants in Kidney Tissue in Healthy Middle-Aged Rats

Abstract

Background & : Both exercise and vitamin D can affect oxidative stress indicators. Despite this, their interaction at the level of kidney tissue has been less investigated. Hence, the main objective of the present study was to determine the effect of vitamin D supplementation and aerobic training on oxidative stress markers in the kidney tissue of middle-aged male rats. Materials and Methods: 20 Wistar male rats (14–12 months and 350–400 g ) were randomly divided into 4 groups: control (CON), aerobic training (EXE), vitamin D (VD) and aerobic training + vitamin D (EXE+VD). Exercise consisted of eight weeks of aerobic training (5 day/week). The VD and EXE+VD groups received vitamin D (500 units/kg) on a daily basis. Olive oil was also used as a placebo. 48 hours after the last intervention session, animal tissues were removed. The gene expression Oxidative stress biomarkers (CAT, Mn-SOD, GPx and XO) were measured by real-time PCR. Data analysis was performed by one-way ANOVA test at the level of P <0.05. Results: The results showed that the EXE+VD increased the gene expression of Mn-SOD, CAT and GPx (P <0.05) and decreased the expression of XO (P <0.05). Also, VD had no effect on the expression of antioxidant enzymes and XO (P>0.05). EXE increased CAT and Mn-SOD expression compared to the CON (P<0.05) and decreased XO expression. Also, CAT values in EXE were significantly higher than VD (P<0.05). Conclusion: According to the results of the present study, it seems that the VD+EXE can increase antioxidant defense and reduce the oxidative stress of kidney tissue.