Vitamin D Status and Its Correlation with Bone Mineral Density in Long Term Survivors After Childhood Hematopoietic Stem Cell Transplantation

Abstract

AbstractAbstract 4472 Introduction:Children undergoing hematopoietic stem cell transplantation (HSCT) are at risk of developing vitamin D deficiency (VDD). However, data on vitamin D status and its correlation with bone mineral density (BMD) in the long term survivors after childhood HSCT is limited. The aim of this study was to determine the prevalence of VDD among long term survivors after HSCT in childhood, and to evaluate the correlations between vitamin D and BMD. Methods:A retrospective study was carried out in patients seen in Long Term follow-up Clinic (LTFC) at our institution from January 2011 to July 2012. Vitamin D deficiency (VDD) and insufficiency (VDI) were defined as serum 25-hydroxyvitamin D (25-OHD) <15 ng/mL and 15–30 ng/mL, respectively. BMD was measured using dual-energy radiograph absorptiometry (Hologic Delphi). Lumbar, total body, and hip BMD Z scores were determined using manufacturer’s normative data based on age. Spearman’s correlation was performed to assess correlation between serum 25-OHD levels and different BMD variables. Results:Ninety eight patients underwent 103 HSCTs between 1990 and 2010. Fifty two (53%) patients were > 5 years out of transplant. A total of 114 vitamin D levels were recorded for the 98 patients, the median 25-OHD level was 26 (range 7 – 68 ng/mL). In 68/114 (60%) observations the 25-OHD levels were less than < 30ng/mL. Of these, 10 (9%) patients had VDD (levels < 15ng/mL, while 58 (51%) had VDI. There were no significant correlations between 25-OHD levels and age at HSCT, gender, underlying diagnosis, type of transplant, or development of acute or chronic GVHD (Table 2). There was a trend towards lower 25-OHD levels after non-TBI based conditioning regimen (p = 0.047).BMD was performed in 83 patients (85%). Low BMD was found in nearly one-third to half of patients tested: 29%, 54%, and 33% of the patients had BMDlumbar, BMDhip and BMDWB Z scores of < −1.0, respectively, while 5%, 9% and 5% of the patients had BMDlumbar, BMDhip and BMDWB Z scores < −2.5, respectively. The median Z scores of the BMDlumbar, BMDhip, and the BMDWB were −0.3 (range −4.2 to 2.4), −1.1 (range −3.3 to 1.9), and −0.4 (range −5.4 to 2.7) respectively. In patients with BMD < −2.5 and < −1.0, the corresponding median 25-OHD was 26 (range 7 – 62 ng/mL) and there was no significant association.Spearman correlation between 25-OHD D level, BMDWB and BMDlumbar showed a correlation coefficient of −0.24 (p value: 0.0409) and −0.22 (p value: 0.0546) respectively. There was no correlation between normal vitamin D levels, VDI and VDD with BMD of the hip, lumbar spine and whole body. Discussion:Low 25-OHD (<30ng/mL) was common (60%) in long term survivors after HSCT during childhood. Similar to other reports, VDD and VDI was seen in 9%, and 51% of the patients respectively. There was only a weak correlation of the 25-OHD levels with BMD of whole body and the lumbar spine, suggesting that factors other than hypovitaminosis D might have contributed to low BMD. There was a small trend of lower 25-OHD levels after non-TBI based conditioning.Table 1Patient characteristicsnSex: male/female57/41Age at HSCT: median (range)5.6 years (1 month-28.4 years)Time from HSCT (years): median (range)5.9 (1–22 years)Current age (years): median (range)16 (4.5–35)Indication for HSCTHematological malignancy52Other malignancy18Non-malignant cause28Conditioning regimenTBI based/non TBI based45/58Myeloablative/non-myeloablative99/4Type of HSCTAutologous/Allogeneic30/73GVHDAcute9 (12%)Chronic10 (14%)Vitamin D Levelsmedian (range)26 (7–68)<1510 (9%)15–3058 (51%)Table 2:Association of Vitamin D levels with variables. TBI- total body irradiation, GVHD- graft versus host disease.VariableTestp-valueSexChi-square test0.0948Age at HSCTWilcoxon rank sum test0.3085Non-TBI ConditioningChi-square test0.0470Final diagnosisFisher's exact test0.5447Type of transplant (autologous or allogeneic)Chi-square test0.6853Acute GVHDFisher's exact test0.6004Chronic GVHDFisher's exact test0.1942 Disclosures:No relevant conflicts of interest to declare.