Vitamin D (Calcitriol) and Interferon-beta at physiological levels act synergistically to modulate cytokine expression in vitro in peripheral blood mononuclear cells.

Abstract

Event Abstract Back to Event Vitamin D (Calcitriol) and Interferon-beta at physiological levels act synergistically to modulate cytokine expression in vitro in peripheral blood mononuclear cells. Niall J. Stewart1*, Steve Simpson2 and Bruce Taylor2 1 University of Tasmania, School of Pharmacy, School of Medicine, Australia 2 University of Tasmania, Menzies Research Institute of Tasmania, Australia Background Current treatments for the autoimmune disease Relapse Remitting Multiple Sclerosis (RRMS) are limited, with Interferon-beta (IFNβ) being one of the most effective. Part of its efficacy is due to its effect on the cytokine profile, suppressing inflammatory cytokines and increasing anti-inflammatory Interleukin-10 (IL-10). Vitamin D is also known to have immunomodulatory effects, therefore we asked whether a combination of IFNβ and calcitriol (the physiologically active form of vitamin D) has synergistic effects on cytokine secretion by peripheral blood mononuclear cells (PBMC) from healthy people. Methods We separated the PBMC from the blood of 22 healthy people, and treated them as follows: stimulated (20ug/ml Concanavilin A); stimulated + 100pg/ml calcitriol; stimulated + 100 IU or 400 IU IFNβ; stimulated + 100pg/ml calcitriol + 100 IU or 400 IU IFNβ. The cytokines IL-2, -4, -6, -10, TNFα, IFNβ and IL-17 were measured flow cytometrically (BD Scientific). Results The majority of subjects were female (59%), and the median serum 25(OH)D was 69.5 nmol/L. Calcitriol alone suppressed IL-2, -4, -6 and 17. IFNβ alone suppressed IL-2, -6 and TNFα, but increased IL-10 four fold. Calctriol and IFNβ acted synergistically to further suppress IL-2, -6, TNFα and IFNγ. No consistent effect of age or serum 25(OH)D on cytokine expression was observed. Significance Co-administration of vitamin D with IFNβ could aid in RRMS by suppressing inflammatory cytokine responses and promoting IL-10. This may also have implications for other inflammatory conditions. Acknowledgements Funding provided by the Royal Hobart Hospital Research Foundation Keywords: Vitamin D, IFNb, Cytokines, PBMC, Immune Modulation Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune-mediated disease pathogenesis Citation: Stewart NJ, Simpson S and Taylor B (2013). Vitamin D (Calcitriol) and Interferon-beta at physiological levels act synergistically to modulate cytokine expression in vitro in peripheral blood mononuclear cells.. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00991 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 26 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Niall J Stewart, University of Tasmania, School of Pharmacy, School of Medicine, Hobart, Australia, nstewart@utas.edu.au Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Niall J Stewart Steve Simpson Bruce Taylor Google Niall J Stewart Steve Simpson Bruce Taylor Google Scholar Niall J Stewart Steve Simpson Bruce Taylor PubMed Niall J Stewart Steve Simpson Bruce Taylor Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.