Vitamin D binding protein (Gc) subtypes by isoelectric focusing and immunoblotting

Abstract

The polymorphism of the human vitamin D binding protein (Gc system) was investigated in a total of 149 sera from unrelated healthy Egyptians residing in Tanta City, Gharbiya Governorate, Nile Delta of Egypt, using isoelectric focusing (IEF) in thin-layer polyacrylamide gel followed by immunoblotting. The estimated gene frequencies were Gc 1S = 0.540, Gc 1F = 0.242 and Gc 2 = 0.218.