Abstract
ABSTRACTBackground:Formation of struvite stones is associated with urinary tract infection by urease-producing bacteria. Biogenic crystal growth in natural and synthetic materials is regulated by the action of inhibitors, ranging from small ions, molecules to large macromolecules.Materials and Methods:We report the dynamics of in vitro crystallization of struvite in presence of vitamin C in synthetic urine using single diffusion gel growth technique. Sodium metasilicate gel of specific gravity 1.05 and the aqueous solution of ammonium dihydrogen phosphate were used as the medium for growing the struvite crystals. The crystallization process was induced by a urease positive struvite stone associated Pseudomonas aeruginosa to mimic the infection leading to stone formation. The grown crystals were characterized by ATR-FTIR and powder XRD. The surface morphology was analysed through FE-SEM for comparison between treatments.Results:We observed decrease in number, dimension, and growth rate of struvite crystals with the increasing concentrations of vitamin C. Crystals displayed well-defined faces and dendritic morphology of struvite in both control and biogenic systems.Conclusion:The results strongly suggest that, vitamin C can modulate the formation of struvite crystals in the presence of uropathogenic bacteria.
Highlights
Struvite or magnesium ammonium phosphate stone is associated with infections by urease-producing bacteria and accounts for about 10-15% of all kidney stones [1]
The bacteria were cultured on tryptic soy broth (TSB) for 18h at 37oC and cells were harvested by centrifugation (6000rpm, 8 min)
In the presence of different concentrations of vitamin C, crystal formation was delayed compared to control
Summary
Struvite or magnesium ammonium phosphate stone is associated with infections by urease-producing bacteria and accounts for about 10-15% of all kidney stones [1]. In addiibju | Vitamin C inhibits crystallization of struvite tion, certain species of Serratia, Corynebacterium and Morganella produce the enzyme urease which can lead to stone formation. Formation of struvite stones is associated with urinary tract infection by urease-producing bacteria. Materials and Methods: We report the dynamics of in vitro crystallization of struvite in presence of vitamin C in synthetic urine using single diffusion gel growth technique. The crystallization process was induced by a urease positive struvite stone associated Pseudomonas aeruginosa to mimic the infection leading to stone formation. Conclusion: The results strongly suggest that, vitamin C can modulate the formation of struvite crystals in the presence of uropathogenic bacteria
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