Abstract

Summary Vitamin B12 and cobalt concentrations were determined in the livers of 4 groups of lambs. Three groups were grazed on cobalt-deficient pastures. Of these groups one received no cobalt treatment and developed marked cobalt deficiency disease. Of the other two groups on cobalt-deficient pastures, one received oral doses of cobalt and the other cobaltic oxide pellets. The fourth group was grazed on cobalttopdressed pastures. Vitamin B12 concentrations in the livers of markedly cobalt-deficient lambs ranged from 0.026 to 0.097 μg/g of wet tissue, with a mean of 0.062 μg/g. Cobalt dosing and cobaltic oxide pellets increased liver vitamin B12 concentrations about fourfold and sevenfold respectively. Vitamin B12 concentrations in the livers of animals from cobalt-top-dressed pasture were of an order similar to those for lambs given cobaltic oxide pellets. Effects of cobalt deficiency disease and of cobalt treatments on B12 Co/total Co ratios are discussed. From all the information available it is conclud...

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