Abstract
Seabuckthorn (Hippophae rhamnoides L.) berries are extremely valuable raw materials for food, pharmaceutical and cosmetic industries. The popularity of the crop is mainly determined by significant amount of biologically active components in the fruit pulp, and by presence of unique lipid fraction in particular, that combines complex of essential fatty acids, carotenoids and tocopherols. At the same time important functional role in the fruits of seabuckthorn belongs to water-soluble vitamins, list of which is represented by a wide range of compounds, including B vitamins. The aim of the current research was to investigate the content of vitamins B in the fruits of various ecotypes of seabuckthorn, growing in the collection of the Lisavenko Research Institute of Horticulture for Siberia, in conditions of forest-steppe area of Altai Territory. Determination of these vitamins was carried out by HPLC.
 As a result the content of thiamine (B1), riboflavin (B2), nicotinic acid (B3) and folic acid (B9) was determined. It was shown that the content of investigated components of seabuckthorn fruit pulp changes during fruit ripening. However, direct dynamics has not been established: both increase and decrease of the content were observed, and in some cases fluctuations during ripening were noted. No significant differences within ecotypes were found.
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