Abstract
A two-step statistical experimental design was employed to optimize the medium for vitamin B 12 production from crude glycerol by Propionibacterium freudenreichii ssp. shermanii. In the first step, using Plackett–Burman design, five of 13 tested medium components (calcium pantothenate, NaH 2PO 4·2H 2O, casein hydrolysate, glycerol and FeSO 4·7H 2O) were identified as factors having significant influence on vitamin production. In the second step, a central composite design was used to optimize levels of medium components selected in the first step. Valid statistical models describing the influence of significant factors on vitamin B 12 production were established for each optimization phase. The optimized medium provided a 93% increase in final vitamin concentration compared to the original medium.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.