Abstract
Intra vitam staining with neutral red dye vividly stains live copepods, providing a rapid method of sorting dead copepods from live. Organisms stained and preserved in acidic media retain coloration for several days, allowing large numbers of organisms to be leisurely sorted following bioassays. Cooling the preserved samples increases color retention. Copepods and cladocerans filter and ingest carmine particles providing an indication of feeding activity. Staining with neutral red at concentrations of 3:200,000 to 1:200,000 is relatively independent of temperature over a range of 5 to 30 C.
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