Abstract
AVolvox species was stained vitally with various dyes. As a result, it was found that the basal hemisphere was especially stainable with aurantia in mother colonies, and with trypan blue in daugher colonies before the natural inversion. When soaked in Bismarck brown solution young daughter colonies are mechanically inverted from the basal pole. This seems to be attributed to the volume decrease of the cavity caused by the dehydration of gelatinous matter which occupies that part. Similar phenomenon is possible to be occurred by use of hypertonic solutions.
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