Abstract
It is difficult to obtain good images of small emulsion polymer particles with an older model SEM which necessitates sputter coating and high accelerating voltage. For the purpose of screening a large number of samples before selecting some for TEM imaging off site, a transmission detector was fitted below the sample holder of an Amray 1600 (Turbo) SEM.Emulsion polymers with particle sizes from 10 to 100 μm were diluted in 1 % phosphotungstic acid (or other negative stain) and applied to Formvar and carbon coated grids. Lacking sophisticated freeze drying equipment, the grids with the sample films were cooled by immersing the stem of the grid holder in liquid nitrogen. They were then freeze dried in vacuo in an old sputter coaterInspite of some inherent difficulties, (such as high beam sensitivity), reasonably good resolution was obtained at 30 KV to magnifications of 150,000x.. Image processing was used, where applicable, to sharpen the images.
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Schedule a callMore From: Proceedings, annual meeting, Electron Microscopy Society of America
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