Abstract
Three-dimensional (3D) in vitro microvasculature in a polydimethylsiloxane-based microdevice was developed as a physiologically relevant model of angiogenesis. The angiogenic process is monitored using stage-top optical coherence tomography (OCT). OCT allows non-invasive monitoring of the 3D structures of the prepared host microvasculature and sprouted neovasculature without fluorescence staining. OCT monitoring takes only a few minutes to scan through the several-millimetre scale range, which provides the advantage of rapid observation of living samples. The obtained OCT cross-sectional images capture 3D features of the angiogenic sprouting process and provide information on the dynamics of luminal formation. The stage-top system used in this study enables the observer to visualize the in vitro dynamics of 3D cultured cells simply and conveniently, offering an alternative monitoring method for studies on angiogenesis and providing quantitative information about vascular morphological changes.
Highlights
Three-dimensional (3D) in vitro microvasculature in a polydimethylsiloxane-based microdevice was developed as a physiologically relevant model of angiogenesis
For the in vitro angiogenesis model, we prepared a microvasculature with endothelial cells using the bottom-up approach via a needle method[9,10]
As the dynamics of the host microvasculature is significant throughout the angiogenic process, microvessels of several sizes (120, 200, and 300 μm in diameter) were monitored
Summary
Three-dimensional (3D) in vitro microvasculature in a polydimethylsiloxane-based microdevice was developed as a physiologically relevant model of angiogenesis. We evaluated stage-top modelled OCT as a convenient alternative method for 3D live imaging of vessel structure change during angiogenesis in vitro. We evaluated dynamic angiogenic sprouting from prepared 3D microvasculatures in a polydimethylsiloxane (PDMS)-based microdevice, with a focus on live monitoring of neovasculature elongation and lumen formation.
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