Abstract
Electron images of biological specimens composed of periodic structures are generally spurious. The reason is that unit structures may superimpose along the direction of view, and a “see through” image is formed especially from negatively stained and unstained objects. Methods to detail features of such objects from two dimensional images included optical and computer processing or combination of optical Fourier transformation and filtering. However, rotational superimposition may be employed when objects have simple symmetry.We described here the use of stroboscope illumination photography (stroboscopy) to demonstrate the distribution of globular units along nucleoprotein fibers of thermally denatured mononucleosomes. The information was further used to predict the structure of intact nucleosomes as seen from bright field (BF) and dark field (DF) electron micrographs.We previously obtained coSnsitometric scans from high resolution DF electron micrographs of partially relaxed unstained nucleosomes isolated from nuclease (EC. 3.1.4.7) digested goose erythrocyte nuclei and showed the presence of high density, periodic, contiguous globular regions (3-4 nm) along the DMA moiety.
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Schedule a callMore From: Proceedings, annual meeting, Electron Microscopy Society of America
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