Visualization of ONOO- and Viscosity in Drug-Induced Hepatotoxicity with Different Fluorescence Signals by a Sensitive Fluorescent Probe.

Abstract

Drug-induced liver injury (DILI) is considered gradually as a serious public health issue, and hepatotoxicity has been regarded as the main clinical problem caused by it. We suspected that both the intracellular viscosity and peroxynitrite (ONOO-) levels in drug-induced hepatotoxicity tissue are higher than those in a healthy liver. For this reason, we have presented a fluorescent probe VO for multichannel imaging viscosity and ONOO- simultaneously. Experimental results showed that VO has satisfactory detection performance for both viscosity and ONOO-, and based on the advantages of its lower cytotoxicity and pH-stabilities, VO was successfully employed to image viscosity and ONOO- in living cells and animals. More importantly, we use the probe to successfully showcase drug-induced hepatotoxicity by imaging viscosity and ONOO- induced by acetaminophen (APAP). All the results indicate that VO has great potential for the detection of viscosity and ONOO- and to assay drug-induced hepatotoxicity. Overall, this work offers a new detection tool/method for a deeper understanding of drug-induced organism injury.