Abstract
Our body has left-right asymmetry of internal organs. In the mouse embryo, left-right asymmetry is established at 8.0 days after fertilization (E 8.0). At E 8.0, the node cavity that has motile cilia appears at the midline of the embryo. The cilia rotate in clockwise direction and generate leftward fluid flow. The leftward flow (nodal flow) breaks symmetry of body by controlling gene expression. In this work, we quantitatively visualized flow field of the nodal flow in wild type and ciliary mutant embryo. PIV analysis reveals that the local fluid flow is sufficient to break left-right symmetry of mouse embryo. This suggests that the node cells have sensitive system for fluid flow. We conclude that PIV is very powerful tool for dissecting biological issues.
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