Abstract
This study has compared two chemically distinct NBD-lipids with regard to their partitioning properties into lateral phases of pure and mixed cholesterol/phosphatidylcholine monolayers. Pure NBD-cholesterol (22-( N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-23,24-bisnor-5-cholen-3-ol), which has the NBD-function in the sterol side chain (at carbon 22), gave a liquid-expanded force-area isotherm on water at 22°C (having a compressibility of 0.005 to 0.007 m/mN), although epifluorescence microscopy of the compressed NBD-cholesterol monolayer revealed that it had a solid-like surface texture. When the compressed NBD-cholesterol monolayer was allowed to expand, it fragmented into large flakes (tens to hundreds of μm in width) which eventually dissolved into a liquid state. The force-area isotherm of pure NBD-phosphatidylcholine (1-hexadecanoyl-2-(12-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)dodecyl- sn-glycero-3-phosphocholine) was also liquid-expanded. When a compressed (30 mN/m) monolayer of NBD-phosphatidylcholine was examined by microscopy, it displayed many bright crystalline spots (about 50 μm across) which appeared to form when the monolayer was allowed to stabilize at this lateral surface pressure. These bright spots disappeared when the monolayer was expanded. When the surface texture of a pure cholesterol monolayer was examined, both probes (at 1 mol%) partitioned very similarly in the sterol monolayer. At low lateral surface pressures (1 and 5 mN/m) the probes appeared to be excluded from the cholesterol phase, forming very bright liquid-like areas against a uniformly black cholesterol phase. At 30 mN/m, NBD-phosphatidylcholine appeared to distribute increasingly into the cholesterol phase, whereas NBD-cholesterol still did not to mix with cholesterol. The characteristic surface texture of the liquid-expanded to liquid-condensed lateral phase transition of pure dipalmitoyl phosphatidylcholine (DPPC) monolayers could be visualized identically with both probes, indicating that these were similarly excluded from the liquid-condensed solid phase of DPPC. Finally, in mixed monolayers containing cholesterol and DPPC (molar ratio 33:67), both probes (at 1 mol%) revealed a similar surface texture of the monolayers (examined at a lateral surface pressure of 0.5 mN/m), suggesting that these partitioned similarly between the different lateral phases present in the mixed monolayer. In conclusion, although the two NBD-probes differed from each other in chemical and physical properties, both acted like ‘impurities’ when admixed into pure or mixed monolayers, and appeared to be equally excluded from lateral phases in which the packing density was high.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
More From: Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.