Abstract
To evaluate whether previously enucleated mouse oocytes that are vitrified are usable for the analysis of human sperm chromosomes. Prospective, comparative laboratory study. Animal breeding and reproduction department in a rural Japan university. Sperm samples were obtained from a fertile donor. Human sperm were injected into previously enucleated-vitrified mouse oocytes. The rates of appearance of the human sperm chromosomes that were injected into previously enucleated-vitrified mouse oocytes. The rates of appearance of human sperm chromosomes were 71%-72% in enucleated-vitrified mouse oocytes. The use of enucleated-vitrified mouse oocytes allowed visualization of human sperm chromosomes.
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