Abstract
The tightly-bound nucleotide of F-actin was replaced with 1, N 6-etheno-adenosine ATP (ADP). An epi-fluorescence optical microscope was modified to visualize efficiently the fluorescent analogue with an excitation-maximum wavelength of 310 nm. This microscope permitted us to visualize single F-actin filaments in solution using the fluorescence of the strongly bound 1, N 6-ethenoadenosine nucleotide. Exchange of the tightly-bound nucleotide of F-actin with a free nucleotide in solution at a high temperature was quantitatively estimated by this method, and the results showed good agreement with results from phosphate release measurements.
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