Abstract
Single-cell RNA imaging enables the detection of endogenous RNA and the analysis of its abundance, localization, and single-cell heterogeneity, which is powerful in the study of RNA biology. G-quadruplex (G4) is a kind of nucleic secondary structure and plays important roles in various biological processes. To reveal the regulating mechanism of monogenic G4s in a specific biological pathway, it is of great important to visualize endogenous RNA G4 in individual genes. Here, we describe Module Assembled Multifunctional Probes Assay (MAMPA) for single-cell visualization of monogenic RNA G4. This method works well in various cell lines and various RNA G4 analytes and has a low detection limit at ten copies per cell.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
