Abstract
The present study employed an imaging mass spectrometry (IMS) method to evaluate the effect of dietary n – 3 fatty acids on the fatty acid composition in rat brain. Rats were divided into two groups and fed either an n – 3 fatty acid-deficient or adequate diet. We determined the decreased n – 3 fatty acids in the hippocampus of rats fed an n – 3 fatty acid-deficient diet compared to the control. IMS visualization was achieved at a resolution of 100 m in rat brain, and showed decreased docosahexaenoic acid (DHA)-containing phosphatidyl choline (PC) or phosphatidyl ethanolamine (PE) in the hippocampus of rats fed an n – 3 fatty acid-deficient diet.
Highlights
Docosahexaenoic acid (DHA, 22:6n – 3) is one of the n – 3 polyunsaturated fatty acids (PUFAs) has important role to our physiological activity
If we visually indicate whether docosahexaenoic acid (DHA)-containing lipids concentrate in the hippocampus, the information helps us to reveal the effect of dietary supplementation of n – 3 PUFAs
We were able to classify the molecular species from exact mass (Me) without tandem mass spectrometry (MS/MS); 3) PUFA lipids were visualized at a resolution of 100 m in the rat brain by imaging mass spectrometry (IMS)
Summary
Docosahexaenoic acid (DHA, 22:6n – 3) is one of the n – 3 polyunsaturated fatty acids (PUFAs) has important role to our physiological activity. We were able to classify the molecular species from exact mass (Me) without tandem mass spectrometry (MS/MS); 3) PUFA lipids were visualized at a resolution of 100 m in the rat brain by IMS. This is the first report to demonstrate decreased n – 3 PUFAs in the rat brain after intake of a PUFA-deficient diet as compared to normal diet
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