Visualization of 14CO2 gas fixation by plants

Ryohei Sugita,Natsuko I Kobayashi,Tomoko M Nakanishi,Keitaro Tanoi

doi:10.1007/s10967-018-6119-3

Abstract

Using the real-time radioisotope imaging system (RRIS), we present the carbon dioxide gas fixation process of a soybean plant applying the 14C-labeled gas. When 14CO2 gas was supplied to the selected mature leaf, the fixed carbon, photosynthate, was transferred and accumulated to the younger leaves preferentially within 24 h. When 14CO2 gas was supplied to the younger leaves, fixed carbon was hardly moved. In the case of the pods, fixed 14CO2 gas in the leaf was preferentially transferred to the closest pod.

Highlights

Carbon constituting the plant tissue is derived from the fixation of the carbon dioxide in air by photosynthesis
Using the real-time radioisotope imaging system (RRIS), we present the carbon dioxide gas fixation process of a soybean plant applying the 14C-labeled gas
When 14CO2 gas was supplied for 30 min to the whole plant grown for 40 days after germination, the imaging plate (IP) image was taken to get the distribution of the 14C-labeled compounds within the plant

Summary

Introduction

Carbon constituting the plant tissue is derived from the fixation of the carbon dioxide in air by photosynthesis. Since photosynthesis is one of the main activities of the plant, many researches were conducted to analyze the mechanism of photosynthesis. There are many studies to analyze the mechanism of photosynthesis, the actual movement of photosynthate in an intact plant was not reported well. To know the movement of the photosynthate in a plant, employment of non-destructive imaging method, which selectively visualizes photosynthate, is necessary. The real-time imaging system using radioisotopes, especially applying radioisotope labeled gas, for plant sample has not been well developed. We have been developing the real-time imaging system, both for macroscopic and microscopic imaging [1,2,3,4,5,6,7,8,9]

Methods

Results

Discussion

Conclusion