Abstract
Elucidation of cell-level transport mediated by vesicles within a living cell provides key information regarding viral infection processes and also drug delivery mechanisms. Although the single-particle tracking method has enabled clear analysis of individual vesicle trajectories, information regarding the entire cell-level intracellular transport is hardly obtainable, due to the difficulty in collecting a large dataset with current methods. In this paper, we propose a visualization method of vesicle transport using optical flow, based on geometric cell center estimation and vector analysis, for measuring the trafficking directions. As a quantitative visualization method for determining the intracellular transport status, the proposed method is expected to be universally exploited in various biomedical cell image analyses.
Highlights
Vesicle refers to the package of intracellular nanoparticles that plays the role of a courier in a living cell [1]
From individual vesicle tracking based on point spread function (PSF) analysis to recent integrated deep-learning approaches [45], various methods have been applied to explain the vesicle movement
The tracking of each vesicle may have revealed the properties of individual vesicle transport, there remained shortcomings in complete understanding of the entire cell-level vesicle movement pattern
Summary
Vesicle refers to the package of intracellular nanoparticles that plays the role of a courier in a living cell [1]. The uptake process, which is known as endocytosis, has been considered to include essential information for understanding the cell signaling and its regulation [3]. Since such information is closely related to elucidating the mechanism of intracellular communication between virus-infected cells and uninfected cells, signal cascades, and drug delivery [4,5], studies have been conducted to reveal the precise movement of vesicles, from the molecular analysis of motor proteins and from the numerical analysis of the vesicle motion itself. Considerable analyses of three-dimensional vesicle trajectories using the single-particle tracking method have been performed from the viewpoint of vesicle-cytoskeleton interaction [10,11,12,13]
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