Abstract
The present study reports the visualization and optical trapping of an individual submicrometer-sized assembly from synthetic polymers using a laser. The assembly was made of PA (palmitic acid) and PEG-A (amino pendant-containing poly(ethylene glycol)) labeled with FITC (fluorescein-5-isothiocyanate type I) at the ratio of PEG-A:FITC = 10:1. The individual submicrometer-sized assemblies were detected at a concentration 50 times lower than the critical aggregation concentration of the PEG-A/PA assembly expected by surface tension measurement. In addition, submicrometer-sized particles were prepared from individual DNA molecules, T4 DNA (166 kbp), in a poly(ethylene glycol) solution. These synthetic assemblies were successfully trapped and transported using a laser, as well as DNA particles. Finally, laser manipulation is shown to be an effective method to judge whether the pair interaction of submicrometer-sized objects is attractive or repulsive.
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